E3 Ligase Family


Protein degradation is a regulated, multistep process. Ubiquitin is loaded onto E1-activating enzymes in an ATP-dependent manner and then transferred to E2-binding enzymes. The same E2 can bind to many E3 ligases, which in turn can ubiquitinate multiple target substrate proteins. E3 ligases bind specific substrate proteins based on substrate degron motifs.


E3 ubiquitin ligases play crucial roles in eukaryotic biology by promoting protein ubiquitination and degradation. Human genome encodes over 600 E3 ubiquitin ligases which could function in distinct cellular contexts, respond to different cellular signals, and process diverse protein targets. In recent years, various E3 ligases became pharmacological targets for developing drugs. Two novel strategies, proteolysis-targeting chimeras (PROTAC) and molecular glue make E3 ligase protein production necessary for structure-based drug design.


The Biortus Protein Catalogue currently offers over 100 of E3 ubiquitin ligases expressed in multiple expression systems, with several structurally resolved and assay developments ready.

 

Catalog No.

Organism

Expression system

Tag

Sequence

BP08867-01A

Homo sapiens

E. coli

His

S2-K592

BP08537-01A

Homo sapiens

E. coli

His

T2-A490

BP11714-02A

Homo sapiens

E. coli

GST

G120-I190

BP12397-02A

Homo sapiens

E. coli

GST

A2-Q228

BP08259-01A

Homo sapiens

E. coli

His

L2-F882

BP13169-13A

Homo sapiens

Insect cell

His/Strep

A2-T344

BP07931-02A

Homo sapiens

E. coli

GST

Y120-P260

BP14090-13A

Homo sapiens

Insect cell

His/Strep

M1-S618

BP08513-01A

Homo sapiens

E. coli

His

S2-E748

BP08523-01A

Homo sapiens

E. coli

His

A2-E870

BP12657-13A

Homo sapiens

Insect cell

His/Strep

S2-T505

BP08514-01A

Homo sapiens

E. coli

His

K2-Y303

BP13911-13A

Homo sapiens

Insect cell

His/Strep

A2-A780

BP07872-01A

Homo sapiens

Insect cell

His

G2-L216

BP07095-01A

Homo sapiens

E. coli

His

S17-N111

BP08972-01A

Homo sapiens

E. coli

His

S2-D474

BP08926-01A

Homo sapiens

E. coli

His

A2-D975

BP13160-13A

Homo sapiens

Insect cell

His/Strep

A2-L600

 

 

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