BirA-O01A
Family ：
Protease and Enzyme
Catalog No. ：
BirA-O01A
Protein ：
birA
UniProt ：
Q8X709
Organism ：
Escherichia coli O157:H7
Expression system ：
E. coli
Tag ：
His
Sequence ：
M1-K321
Assay ：
Structure ：
Inquiry ：
是
Product Information
Symbol/Gene Name: birA
Alias: Bifunctional ligase/repressor BirA,Biotin operon repressor,Biotin--[acetyl-CoA-carboxylase] ligase,Biotin--protein ligase,Biotin-[acetyl-CoA carboxylase] synthetase
Uniprot: Q8X709
Background: Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
Construct: N-6His-BirA
Expression Host: E. coli
Purity: 90%
Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
Supplied as sterile
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Endotoxin:
< 0.01 EU per μg of the protein
Shipping:
Liquid. It is shipped out with blue ice.
Stability & Storage:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recombinant protein