BP470A1-24A

Family :

CDK

Catalog No. :

BP470A1-24A

Protein :

CDK3/Cyclin E1

UniProt :

Q00526/P24864

Organism :

Homo sapiens

Expression system :

Insect cell

Tag :

GST/Flag/His

Sequence :

M1-H305/M1-E410

Inquiry :

Symbol/Gene Name:  CDK3;CCNE1

Alias:  Cyclin-dependent kinase 3,Cell division protein kinase 3;G1/S-specific cyclin-E1

Uniprot: Q00526/P24864

Background: Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.

Essential for the control of the cell cycle at the G1/S (start) transition.

Construct: N-GST-3C-CDK3(1-305end)/pFastBac1-Flag-6His-TEV-Cyclin E1(1-410)

Expression Host: Insect cell

Purity: 90%

Formulation:

Supplied as sterile 50 mM HEPES (pH 7.5), 150 mM NaCl, 5% glycerol.

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.