BP04977-00A

Family :

Protease and Enzyme

Catalog No. :

BP04977-00A

Protein :

kshB

UniProt :

P9WJ93

Organism :

Mycobacterium tuberculosis

Expression system :

E. coli

Tag :

No tag

Sequence :

M1-E358

Inquiry :

Symbol/Gene Name:  hmp

Alias:  3-ketosteroid-9-alpha-monooxygenase, ferredoxin reductase component, 3-ketosteroid-9-alpha-hydroxylase, ferredoxin reductase component, KSH, Androsta-1, 4-diene-3, 17-dione 9-alpha-hydroxylase, Rieske-type oxygenase, RO

Uniprot: P9WJ93

Background: Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A. KSH is also able to use 4-androstene-3,17-dione (AD), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4-BNC), 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate(4-BNC), 3-oxo-23,24-bisnorcholesta-4-en-22-oyl-coenzyme A thioester (4-BNC-CoA) and 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A thioester(4-BNC-CoA) as substrates.

Construct: GG-KSHB(M1-E358 end) (TB enzyme)

Expression Host: E. coli

Purity: 90%

Formulation: 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.