BP14282-13A

Family :

Protease and Enzyme

Catalog No. :

BP14282-13A

Protein :

MGMT

UniProt :

P16455

Organism :

Homo sapiens

Expression system :

E. coli

Tag :

His/Strep

Sequence :

M1-N207

Inquiry :

Symbol/Gene Name:  MGMT

Alias:  Methylated-DNA--protein-cysteine methyltransferase,6-O-methylguanine-DNA methyltransferase,MGMT,O-6-methylguanine-DNA-alkyltransferase

Uniprot: P16455

Background: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Construct: 6His-strepII-TEV-GG-MGMT(M1-N207 end)

Expression Host: E. coli

Purity: 90%

Formulation: 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.