otsA-O00A
Family ：
Protease and Enzyme
Catalog No. ：
otsA-O00A
Protein ：
otsA
UniProt ：
P9WN11
Organism ：
Mycobacterium tuberculosis
Expression system ：
E. coli
Tag ：
No tag
Sequence ：
M1-G500
Assay ：
Structure ：
Inquiry ：
是
Symbol/Gene Name: otsA
Alias: Trehalose-6-phosphate synthase,TPS,Alpha,alpha-trehalose-phosphate synthase [UDP-forming],Osmoregulatory trehalose synthesis protein A,OtsA
Uniprot: P9WN11
Background: Involved in the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P), and probably in the osmoprotection via the biosynthesis of trehalose (PubMed:12473104, PubMed:27513637). Catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate (PubMed:12473104). Is specific for the glucosyl acceptor (Glc-6-P cannot be replaced by either mannose-6-P, fructose-6-P or glucosamine-6-P), but any of the glucose sugar nucleotides can be used as glucosyl donors (PubMed:12473104). It is more active with the purine sugar nucleotides than with the pyrimidine sugar nucleotides (PubMed:12473104).
Construct: GG-otsA(M1-G500 end) (TB enzyme)
Expression Host: E. coli
Purity: 90%
Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
Supplied as sterile
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Endotoxin:
< 0.01 EU per μg of the protein
Shipping:
Liquid. It is shipped out with blue ice.
Stability & Storage:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recombinant protein