BP01293-00A

Family :

Protease and Enzyme

Catalog No. :

BP01293-00A

Protein :

COLAER_02088

UniProt :

A4ECA9

Organism :

Collinsella aerofaciens

Expression system :

E. coli

Tag :

No tag

Sequence :

T189V,V207M

Inquiry :

Symbol/Gene Name:  

Alias:  7beta-hydroxysteroid dehydrogenase,7beta-HSDH,NADP-dependent 7beta-hydroxysteroid dehydrogenase

Uniprot: A7B4V1

Background: 7beta-hydroxysteroid dehydrogenase that catalyzes the reduction of the 7-oxo group of 7-oxo-lithocholate (7-oxo-LCA), to yield ursodeoxycholate (UDCA). As R.gnavus is a common core bacterium of the human gut microbiota, this enzyme contributes to the formation of UDCA in the human colon. UDCA is regarded as a chemopreventive beneficial secondary bile acid due to its low hydrophobicity; it protects hepatocytes and bile duct epithelial cells against necrosis and apoptosis induced by more hydrophobic secondary bile acids like deoxycholate (DCA). This enzyme is also able to catalyze the reverse reaction in vitro, i.e. the oxidation of the 7beta-hydroxy group of UDCA to 7-oxo-LCA, but much less efficiently than the reduction reaction.

Construct: 7β HSDH(T189V,V207M)

Expression Host: E. coli

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.