hSSTR2/GNAI1/GNB1-H411A
Family ：
GPCR
Catalog No. ：
hSSTR2/GNAI1/GNB1-H411A
Protein ：
SSTR2-Gi
UniProt ：
P30874; P63096; P62873; P59768
Organism ：
Homo sapiens
Expression system ：
Insect cell
Tag ：
His
Sequence ：
Inquire for more details
Assay ：
Structure ：
Inquiry ：
是
Symbol/Gene Name: SSTR2;GNAI1;GNB1;GNG2
Alias: Somatostatin receptor type 2,SS-2-R,SS2-R,SS2R,SRIF-1;Guanine nucleotide-binding protein G(i) subunit alpha-1,Adenylate cyclase-inhibiting G alpha protein;Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,Transducin beta chain 1;Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2,G gamma-I
Uniprot: P30874; P63096; P62873; P59768
Background: Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. Inhibits calcium entry by suppressing voltage-dependent calcium channels. Acts as the functionally dominant somatostatin receptor in pancreatic alpha- and beta-cells where it mediates the inhibitory effect of somatostatin-14 on hormone secretion. Inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. Stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. Mediates negative regulation of insulin receptor signaling through PTPN6. Inactivates SSTR3 receptor function following heterodimerization.
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Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (By similarity). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed:17635935). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed:22327364).
Construct: Flag-hSSTR2(M1-I369 end)-TEV-10His/GNAI1(M1-F354 end) (S47N, G203A, E245A, A326S)/8His-TEV-GNB1(M1-N340 end)/GNG2(M1-L71 end)
Expression Host: Insect cell
Purity: 90%
Formulation: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.00075% (w/v) LMNG, 0.00015% (w/v) CHS, 0.1 mM TCEP, 1 μM SSP-008
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Endotoxin:
< 0.01 EU per μg of the protein
Shipping:
Liquid. It is shipped out with blue ice.
Stability & Storage:
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recombinant protein