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纯化工艺
- 分类:蛋白制备
- 发布时间:2020-08-14 00:00:00
- 访问量:0
概要:Protein Purification
With a total of 10 AKTA FPLC systems and a great selection (affinity, ion exchange, reverse phase, hydrophobic interaction, and size exclusion) of purification columns, Biortus Biology Department is well-equipped for purification of macromolecular biomolecules. Below shows our general purification scheme for most proteins
Wuxi Biortus Biosciences Co. Ltd.
We purify hundreds of different proteins per year. The scheme works fine for most of them. We also have other strategies in place for “difficult” proteins.
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我们有超过20台AKTA FPLC系统和丰富的纯化柱选择(亲和力,离子交换,反相,疏水作用和尺寸排阻)和配套的质控设备,包括LC-MS,SEC-MALs,佰翱得的纯化部门设备完善,可纯化大分子生物蛋白和复合物,并鉴定分子量,纯度,均一性和修饰情况。 下面显示了我们对大多数蛋白质的常规纯化方案:
我们每年都要纯化上百种不同的蛋白。以上的纯化策略适合于大多数蛋白,当然我们在纯化一些难度较高的蛋白时会用其他的一些策略。