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CellTiter-Glo®发光和MTT细胞活力测定

CellTiter-Glo®发光和MTT细胞活力测定

概要:The cell culture facility used for recombinant protein production has also allowed our in vitro assays team to develop cell-based assays here in Biortus. Together with the amplitude of plate readers available, we could develop and perform many cell-based assays for our clients. There are two types of cell-based cell cytotoxicity assays that we do. The CellTiter-Glo® luminescent cell viability assay and the widely used MTT cell proliferation/cytotoxicity assay. The former is a convenient assay that does not require cell washing and removal of cell medium and measures luminescence generated from the amount of ATP provided by metabolically active cells. The latter measures the reduction of MTT by metabolically active cells detected by increase in 570nm absorbance.
概要:The cell culture facility used for recombinant protein production has also allowed our in vitro assays team to develop cell-based assays here in Biortus. Together with the amplitude of plate readers available, we could develop and perform many cell-based assays for our clients. There are two types of cell-based cell cytotoxicity assays that we do. The CellTiter-Glo® luminescent cell viability assay and the widely used MTT cell proliferation/cytotoxicity assay. The former is a convenient assay that does not require cell washing and removal of cell medium and measures luminescence generated from the amount of ATP provided by metabolically active cells. The latter measures the reduction of MTT by metabolically active cells detected by increase in 570nm absorbance.
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结合酶标仪的多种功能,佰翱得还可以为客户开发基于细胞的检测方法。 我们有两种类型的细胞毒性测试方法,CellTiter-Glo®化学发光细胞活力检测方法和MTT细胞增殖/细胞毒性检测方法。前者是检测比较简单,不需要细胞洗涤和去除细胞培养基,定量检测活细胞代谢产生的ATP所产生的化学发光信号。后者基于活细胞能通过代谢减少MTT的原理从而检测到570nm吸光度的增加。

 

佰翱得细胞活性分析实例:CellTiter-Glo® (PC-9细胞) 和 MTT (H1975 细胞)

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